P-MCL004

Experiment PMCL004V. Menachery and A. SchaeferKawaoka 13-18PublishedView All Experiments
MERS-CoV in Calu-3 cells; ChIP analyses
Perturbations
Infection
Viruses
Mers-CoV
Species
Human
Cell/Tissue Types
Calu-3
Time Points
18 h
Uploaded
ChIP
N/A
NFKB Expression, IFNB Expression, ISRE Expression, Experiments

Purpose:  To obtain samples for ChIP analysis using the following viruses:

  • infectious clone-derived Middle Eastern Respiratory Syndrome Coronavirus

Overview of Experiment:

Time Points: 18 h post-infection

Replicates:  3 replicate samples were collected for each condition (each replicate consists of 3 x 6-wells combined)

Mocks: Time-matched

Cells: Calu-3 cells (human bronchial epithelial cells)

Multiplicity of Infection (MOI): 5 PFU per cell

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