P-ICL105

Experiment PICL105Kevin WaltersKawaoka 13-18PublishedMarch 24, 2015View All Experiments
H5N1 in Calu-3 cells; ChIP analyses
Perturbations
Infection
Viruses
Influenza
Species
Human
Cell/Tissue Types
Calu-3
Time Points
4 time points (0, 12, 18 and 24 h); 4 time points available for ChIP analysis
Uploaded
Design, Workbook, Viral Titers, ChIP
N/A
NFKB Expression, IFNB Expression, ISRE Expression, Experiments
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Purpose:  To obtain samples for ChIP analysis using the following viruses:

  • reverse genetics-derived A/Vietnam/1203/2004 (H5N1)

Overview of Experiment:

Time Points: 0, 12, 18 and 24 h post-infection

Replicates:  3 replicate samples were collected for each condition (each replicate consists of 3 x 6-wells combined)

Mocks: Time-matched

Cells: Calu-3 cells (human bronchial epithelial cells)

Multiplicity of Infection (MOI): 1 PFU per cell

Additional samples: Collected supernatants from each well for virus titration